Reporter

Part:BBa_I715034:Design

Designed by: Andrew Martens   Group: iGEM07_Davidson_Missouri_W   (2007-07-20)


pLAC-RBS-RFP1-HixC-RFP2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 824
    Illegal AgeI site found at 936
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that, to avoid a frameshift, HixCRFP2 was designed such that it has an extra nucleotide between the HixC site and the RFP2 section.


Source

Ligated BBa_S03738 with BBa_S03740.

References